Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.

@article{Chatterjee2007BiosynthesisOT,
  title={Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.},
  author={Abhishek Chatterjee and Christopher T. Jurgenson and Frank C Schroeder and Steven E. Ealick and Tadhg P Begley},
  journal={Journal of the American Chemical Society},
  year={2007},
  volume={129 10},
  pages={2914-22}
}
Thiazole synthase catalyzes the formation of the thiazole moiety of thiamin pyrophosphate. The enzyme from Saccharomyces cerevisiae (THI4) copurifies with a set of strongly bound adenylated metabolites. One of them has been characterized as the ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid. Attempts toward yielding active wild-type THI4 by releasing protein-bound metabolites have failed so far. Here, we describe the identification and characterization of two partially… CONTINUE READING

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Structural insights into the function of the thiamin biosynthetic enzyme Thi 4 from Saccharomyces cere V isiae

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  • Biochemistry
  • 2006

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