Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).

@article{Park2003BiosynthesisOT,
  title={Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).},
  author={J. Park and Pieter C. Dorrestein and Huili Zhai and Cynthia L Kinsland and Fred W. McLafferty and Tadhg P Begley},
  journal={Biochemistry},
  year={2003},
  volume={42 42},
  pages={12430-8}
}
While most of the proteins required for the biosynthesis of thiamin pyrophosphate have been known for more than a decade, the reconstitution of this biosynthesis in a defined biochemical system has been difficult due to the novelty of the chemistry involved. Here we demonstrate the first successful enzymatic synthesis of the thiazole moiety of thiamin from glycine, cysteine, and deoxy-D-xylulose-5-phosphate using overexpressed Bacillus subtilis ThiF, ThiS, ThiO, ThiG, and a NifS-like protein… CONTINUE READING