Biosynthesis of the insulin receptor in rat adipose cells. Intracellular processing of the Mr-190 000 pro-receptor.

@article{Hedo1985BiosynthesisOT,
  title={Biosynthesis of the insulin receptor in rat adipose cells. Intracellular processing of the Mr-190 000 pro-receptor.},
  author={J. A. Hedo and Ian A. Simpson},
  journal={The Biochemical journal},
  year={1985},
  volume={232 1},
  pages={71-8}
}
We investigated the biosynthesis of the insulin receptor in primary cultures of isolated rat adipose cells. Cells were pulse-chase-labelled with [3H]mannose, and at intervals samples were homogenized. Three subcellular membrane fractions were prepared by differential centrifugation: high-density microsomal (endoplasmic-reticulum-enriched), low-density… CONTINUE READING