Corpus ID: 25459793

Biosynthesis of tetrahydrobiopterin in the presence of dihydrofolate reductase inhibitors.

@article{Duch1983BiosynthesisOT,
  title={Biosynthesis of tetrahydrobiopterin in the presence of dihydrofolate reductase inhibitors.},
  author={D. Duch and C. Lee and M. Edelstein and C. A. Nichol},
  journal={Molecular pharmacology},
  year={1983},
  volume={24 1},
  pages={
          103-8
        }
}
Since there is no nutritional requirement for the biopterin cofactor, we attempted to create a drug-induced deficiency in rats in order to study the role of tetrahydrobiopterin in regulating the biosynthesis of dopamine and serotonin. The hypothesis that dihydrofolate reductase (EC 1.5.1.3) mediates the final step in the de novo synthesis of tetrahydrobiopterin was tested by treating rats with methotrexate along with leucovorin as a protective agent; there was no reduction in total biopterin or… Expand
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