Biosynthesis of soluble carotenoid holoproteins in Escherichia coli

  title={Biosynthesis of soluble carotenoid holoproteins in Escherichia coli},
  author={C{\'e}line Bourcier de Carbon and Adrien Thurotte and Adj{\'e}l{\'e} Wilson and François Perreau and Diana Kirilovsky},
  booktitle={Scientific reports},
Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo… CONTINUE READING
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