Biosynthesis of proteoglycans by rat embryo parietal yolk sacs in organ culture.

@article{Iozzo1986BiosynthesisOP,
  title={Biosynthesis of proteoglycans by rat embryo parietal yolk sacs in organ culture.},
  author={Renato V Iozzo and C C Clark},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 15},
  pages={
          6658-69
        }
}
The embryonic rat parietal yolk sac has been previously shown to synthesize a number of basement membrane glycoconjugates including type IV procollagen, laminin, and entactin. In this study, parietal yolk sacs were isolated from 14.5-day rat embryos and incubated in organ culture for 4-7 h with [35S]sulfate, [3H] glucosamine, and/or 3H-labeled amino acids, and the newly synthesized proteoglycans were characterized. The major [35S]sulfate-labeled macromolecule represented approximately 90% of… CONTINUE READING