Biosynthesis of new indigoid inhibitors of protein kinases using recombinant cytochrome P450 2A6.

@article{Wu2005BiosynthesisON,
  title={Biosynthesis of new indigoid inhibitors of protein kinases using recombinant cytochrome P450 2A6.},
  author={Zhongliu-Liu Wu and Pramod Aryal and Olivier Lozach and Laurent Meijer and F Peter Guengerich},
  journal={Chemistry & biodiversity},
  year={2005},
  volume={2 1},
  pages={51-65}
}
Glycogen synthase kinase-3 (GSK-3) is a potential drug target for a number of human diseases. Some indigoids have been found to be potent inhibitors of GSK-3, and individual compounds with better activity, specificity, and solubility are desired. In this work, a new disubstituted indigoid generation system was developed with a tryptophanase-deficient Escherichia coli strain as a host to express the human cytochrome P450 2A6 mutant L240C/N297Q, which catalyzes the oxidation of indole to isatin… CONTINUE READING