Biosynthesis of membrane polypeptides of rat liver peroxisomes.

@article{Suzuki1987BiosynthesisOM,
  title={Biosynthesis of membrane polypeptides of rat liver peroxisomes.},
  author={Yota Suzuki and Tadao Orii and Masaki Takiguchi and Michio Mori and Mamoru Hijikata and Tamae Hashimoto},
  journal={Journal of biochemistry},
  year={1987},
  volume={101 2},
  pages={
          491-6
        }
}
The biosynthesis of three major peroxisomal membrane polypeptides of rat liver was investigated. Total hepatic RNA extracted by the guanidinium/CsCl method from three control and three di(2-ethylhexyl)phthalate (a peroxisomal proliferator)-fed rats was translated in vitro in a rabbit reticulocyte lysate protein-synthesizing system. Translation products were immunoprecipitated by the antibodies against peroxisomal membrane polypeptides, subjected to sodium dodecyl sulfate/polyacrylamide gel… CONTINUE READING

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