Biosynthesis of indole-3-acetic acid in protoplasts, chloroplasts and a cytoplasmic fraction from barley (Hordeum vulgare L.)

Abstract

Protoplast preparations from barley (Hordeum vulgare L.) enzymatically converted [5-3H]tryptophan to [3H]indole-3-acetic acid (IAA). Both a chloroplast and a crude cytoplasmic fraction, isolated from protoplasts that had previously been fed [5-3H]tryptophan, contained [3H]IAA. Chloroplast and cytoplasmic preparations, isolated from protoplasts and thereafter incubated with [5-3H]tryptophan, also synthesized [3H]IAA, although, in both instances the pool size was less than 50% of that detected in the in-vivo feeds. There were no significant differences in the amounts of [3H]IAA that accumulated in protoplast and chloroplast preparations incubated in light and darkness.

DOI: 10.1007/BF00392778

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Cite this paper

@article{Sandberg1982BiosynthesisOI, title={Biosynthesis of indole-3-acetic acid in protoplasts, chloroplasts and a cytoplasmic fraction from barley (Hordeum vulgare L.)}, author={Goran Sandberg and Einar H. Jensen and Alan Crozier}, journal={Planta}, year={1982}, volume={156}, pages={541-545} }