Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin.

  title={Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin.},
  author={Kristien Tytgat and Hans A. B{\"u}ller and Frank J. M. Opdam and Yeong Shik Kim and Alexandra W. C. Einerhand and Jan Dekker},
  volume={107 5},
BACKGROUND/AIMS Human colonic epithelium produces large amounts of mucin. The aim of this study was to examine mucin biosynthesis in the human colon. METHODS Human colonic mucin was isolated using CsCl density gradients, and polyclonal antiserum was raised. Biosynthesis of colonic mucins was studied by labeling colonic explants with 35S-labeled amino acids or [35S]sulfate and subsequent immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS The… CONTINUE READING
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