Biosynthesis of glycosylated human lysozyme mutants.

@article{Horst1991BiosynthesisOG,
  title={Biosynthesis of glycosylated human lysozyme mutants.},
  author={Miles Horst and Nicole Syringa Harth and Andrej Hasilik},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 21},
  pages={13914-9}
}
Complementary DNA encoding human lysozyme was subjected to oligonucleotide-directed mutagenesis. At one of three selected positions, amino acid residues 22, 68, or 118, the signal for N-linked glycosylation was created. The mutant DNAs were inserted into a eucaryotic vector and transfected into cultured hamster cells. The three mutant cDNAs directed synthesis of lysozyme mutants, which were named LI, LII, and LIII. The mutant lysozymes LI and LII comprised mixtures of glycosylated and… CONTINUE READING
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