Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain.

@article{Watanabe1997BiosynthesisOA,
  title={Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain.},
  author={Masato Watanabe and Michinori Matsuo and Seigo Tanaka and Hiroshi Akimoto and Satoru Asahi and Shin-Ichiro Nishimura and Jon R. Katze and Takeshi Hashizume and Pamela F. Crain and James A. McCloskey and Norihiro Okada},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 32},
  pages={20146-51}
}
Archaeosine is a novel derivative of 7-deazaguanosine found in transfer RNAs of most organisms exclusively in the archaeal phylogenetic lineage and is present in the D-loop at position 15. We show that this modification is formed by a posttranscriptional base replacement reaction, catalyzed by a new tRNA-guanine transglycosylase (TGT), which has been isolated from Haloferax volcanii and purified nearly to homogeneity. The molecular weight of the enzyme was estimated to be 78 kDa by SDS-gel… CONTINUE READING

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