Biosynthesis of a Thiamin Antivitamin in Clostridium botulinum

@article{Cooper2014BiosynthesisOA,
  title={Biosynthesis of a Thiamin Antivitamin in Clostridium botulinum},
  author={Lisa E. Cooper and Se{\'a}n E. O’Leary and Tadhg P. Begley},
  journal={Biochemistry},
  year={2014},
  volume={53},
  pages={2215 - 2217}
}
Bacimethrin-derived 2′-methoxythiamin pyrophosphate inhibits microbial growth by disrupting metabolic pathways dependent on thiamin-utilizing enzymes. This study describes the discovery of the bacimethrin biosynthetic gene cluster of Clostridium botulinum A ATCC 19397 and in vitro reconstitution of bacimethrin biosynthesis from cytidine 5′-monophosphate. 
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