Biosynthesis of NAAG by an enzyme‐mediated process in rat central nervous system neurons and glia

@article{Gehl2004BiosynthesisON,
  title={Biosynthesis of NAAG by an enzyme‐mediated process in rat central nervous system neurons and glia},
  author={Laura M Gehl and O H Saab and Tomasz Bzdȩga and B. Wr{\'o}blewska and Joseph H Neale},
  journal={Journal of Neurochemistry},
  year={2004},
  volume={90}
}
The peptide transmitter N‐acetylaspartylglutamate (NAAG) is present in millimolar concentrations in mammalian spinal cord. Data from the rat peripheral nervous system suggest that this peptide is synthesized enzymatically, a process that would be unique for mammalian neuropeptides. To test this hypothesis in the mammalian CNS, rat spinal cords were acutely isolated and used to study the incorporation of radiolabeled amino acids into NAAG. Consistent with the action of a NAAG synthetase… 
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TLDR
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TLDR
Results indicate that synaptically released NAAG, as well as that which may be released from glia, is removed from the extracellular space by direct uptake aswell as the robust enzymatic degradation of the peptide.
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TLDR
NAAG is present in a subpopulation of primary afferent spinal neurons and that its biosynthesis is mediated by a dipeptide synthetase, suggesting a precursor role for the large N‐acetylaspartate pool.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
These data are the first which demonstrate the release of NAAG following biosynthesis from a radiolabeled precursor and are consistent with synaptic release of this dipeptide.
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TLDR
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