Biosynthesis of Entamoeba histolytica proteophosphoglycan in vitro.

  title={Biosynthesis of Entamoeba histolytica proteophosphoglycan in vitro.},
  author={Ranjana Singh Arya and Alka Mehra and Sudha Bhattacharya and Ram A Vishwakarma and Alok Bhattacharya},
  journal={Molecular and biochemical parasitology},
  volume={126 1},
A complex glycoconjugate proteophosphoglycan (PPG) is present on the surface of the pathogenic protozoan parasite Entamoeba histolytica but not in the non-pathogenic Entamoeba dispar. It is thought to be an important molecule involved in pathogenesis. In order to study its biosynthesis, an in vitro cell-free system was developed. The specificity of the system was demonstrated by various criteria including immunoprecipitation by a specific monoclonal antibody. The in vitro synthesized molecule… CONTINUE READING