Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities.

@article{Luesch2003BiosynthesisO4,
  title={Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities.},
  author={H. Luesch and D. Hoffmann and J. Hevel and Julia E Becker and T. Golakoti and R. Moore},
  journal={The Journal of organic chemistry},
  year={2003},
  volume={68 1},
  pages={
          83-91
        }
}
The biosynthesis of the unusual amino acid 4-methylproline in the Nostoc genus of cyanobacteria was investigated on the genetic and enzymatic level. Two genes involved in the biosynthesis were cloned and the corresponding enzymes, a zinc-dependent long-chain dehydrogenase and a Delta(1)-pyrroline-5-carboxylic acid (P5C) reductase homologue, were overexpressed in Escherichia coli and biochemically characterized. Putative substrates were synthesized to test enzyme substrate specificities, and… Expand
64 Citations
Natural product biosyntheses in cyanobacteria: A treasure trove of unique enzymes
  • 95
  • PDF
Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis
  • 5
Phylogenetic analysis of type I polyketide synthase and nonribosomal peptide synthetase genes in Antarctic sediment
  • 45
Distribution and Diversity of Natural Product Genes in Marine and Freshwater Cyanobacterial Cultures and Genomes
  • 117
  • Highly Influenced
  • PDF
...
1
2
3
4
5
...