Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities.

@article{Luesch2003BiosynthesisO4,
  title={Biosynthesis of 4-methylproline in cyanobacteria: cloning of nosE and nosF genes and biochemical characterization of the encoded dehydrogenase and reductase activities.},
  author={Hendrik Luesch and Dietmar Hoffmann and Joan M Hevel and Julia E. Becker and Trimurtulu Golakoti and Richard Eugene Moore},
  journal={The Journal of organic chemistry},
  year={2003},
  volume={68 1},
  pages={
          83-91
        }
}
The biosynthesis of the unusual amino acid 4-methylproline in the Nostoc genus of cyanobacteria was investigated on the genetic and enzymatic level. Two genes involved in the biosynthesis were cloned and the corresponding enzymes, a zinc-dependent long-chain dehydrogenase and a Delta(1)-pyrroline-5-carboxylic acid (P5C) reductase homologue, were overexpressed in Escherichia coli and biochemically characterized. Putative substrates were synthesized to test enzyme substrate specificities, and… CONTINUE READING

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