Biosynthesis of (-)-Kaurene in Cell-free Extracts of Immature Pea Seeds.

  title={Biosynthesis of (-)-Kaurene in Cell-free Extracts of Immature Pea Seeds.},
  author={J. A. D. W. Anderson and Thomas C. Moore},
  journal={Plant physiology},
  volume={42 11},
Mevalonate-(14)C was incorporated into (-)-kaurene-(14)C in cell-free extracts of immature pea (Pisum sativum L.) seeds. The identification of (14)C-product as (-)-kaurene was based on: A) comparison with authentic (-)-kaurene on thin-layer and gas-liquid chromatography; and B) oxidation of (14)C-product and (-)-kaurene with osmium tetroxide to form the common derivative kaurane-16,17-diol. The enzyme system is heat labile and is dependent upon ATP and Mg(2+) or Mn(2-), with Mn(2+) being a more… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 14 extracted citations

Similar Papers

Loading similar papers…