Biosynthesis and secretion of a precursor of nisin Z by Lactococcus lactis, directed by the leader peptide of the homologous lantibiotic subtilin from Bacillus subtilis.

@article{Kuipers1993BiosynthesisAS,
  title={Biosynthesis and secretion of a precursor of nisin Z by Lactococcus lactis, directed by the leader peptide of the homologous lantibiotic subtilin from Bacillus subtilis.},
  author={Oscar P. Kuipers and Harry S. Rollema and Willem M de Vos and Roland J. Siezen},
  journal={FEBS letters},
  year={1993},
  volume={330 1},
  pages={23-7}
}
The DNA sequence encoding the leader peptide of the lantibiotic subtilin from Bacillus subtilis was fused to the sequence encoding pronisin Z, and this hybrid gene was expressed in a Lactococcus lactis strain that produces nisin A. This strain simultaneously secreted nisin A and a protein of approximately 6 kDa. Amino acid sequencing of the purified 6 kDa protein and structural analysis of its main tryptic fragment by two-dimensional 1H-NMR showed that it consists of the unmodified leader… CONTINUE READING

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Nisin and Novel Lantibiotics (Jung, G., and Sahl

  • G. Jung
  • H.-G., Eds.) pp. l-31. ESCOM. Leiden
  • 1991
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