Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells.

@article{Moremen1985BiosynthesisAM,
  title={Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells.},
  author={Kelley W Moremen and Oscar Touster},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 11},
  pages={6654-62}
}
The biosynthesis and post-translational modification of mannosidase II, an enzyme required in the maturation of asparagine-linked oligosaccharides in the Golgi complex, has been investigated. Antibody raised against this enzyme purified from rat liver Golgi membranes was used to immunoprecipitate mannosidase II from rat liver, 3T3 cells, or HeLa cells. Mannosidase II immunoprecipitated from rat liver Golgi membranes, when analyzed by polyacrylamide gel electrophoresis, migrated with an apparent… CONTINUE READING

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