• Biology, Medicine
  • Published in
    The Journal of biological…
    1986

Biosynthesis and intracellular transport of rat liver 5'-nucleotidase.

@article{Wada1986BiosynthesisAI,
  title={Biosynthesis and intracellular transport of rat liver 5'-nucleotidase.},
  author={Ikuo Wada and Masaru Himeno and Koji Furuno and Koichi Kato},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 5},
  pages={
          2222-7
        }
}
To investigate biosynthesis and intracellular transport of 5'-nucleotidase, we purified this enzyme from rat liver and prepared antibodies. In immunoblot analysis, 5'-nucleotidase from the plasmalemmal, Golgi, and tritosomal fractions migrated as a single band of 72 kDa. The immunoreactive 68-kDa band was detected in the rough microsomal fraction and only the 72-kDa polypeptide contained sialic acids. The single polypeptide of 61 kDa immunoprecipitated from the translation products with the… CONTINUE READING

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Seikuguku 52 , 674 146 , 625 - 633 345 , 180 - 197 6947 -

S. Eto, K. Kato, Y. Ikehara
  • 1980