Biosynthesis, development, and regulation of neuropeptide Y in superior cervical ganglion culture.

@article{Marek1989BiosynthesisDA,
  title={Biosynthesis, development, and regulation of neuropeptide Y in superior cervical ganglion culture.},
  author={Kenneth L Marek and Richard E Mains},
  journal={Journal of neurochemistry},
  year={1989},
  volume={52 6},
  pages={1807-16}
}
The biosynthesis of neuropeptide Y (NPY) and norepinephrine (NE) has been examined in dissociated neuronal cultures from newborn rat superior cervical ganglion (SCG). NPY synthetic rate was measured by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after incubation in medium containing a labeled amino acid. The authenticity of the NPY was confirmed by reverse-phase HPLC analyses of tryptic peptides. The NPY synthetic rate in cultures grown in complete serum… CONTINUE READING

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