Biosynthesis, biotechnological production, and application of teicoplanin: current state and perspectives

  title={Biosynthesis, biotechnological production, and application of teicoplanin: current state and perspectives},
  author={Hyung-Moo Jung and M. Jeya and Sang-Yong Kim and Hee-Jung Moon and Raushan Kumar Singh and Ye-Wang Zhang and Jung-Kul Lee},
  journal={Applied Microbiology and Biotechnology},
The glycopeptide teicoplanin isolated from the fermentation broth of Actinoplanes teichomyceticus is used to treat serious Gram-positive bacterial infections that are resistant to other antibiotics, e.g. β-lactams. The long time frame and progressively broader clinical use of teicoplanin has eventually led to the emergence and spreading of resistance in enterococci and staphylococci towards the antibiotics. Given the structural complexity of the natural product, only fermentative routes are… 

Manipulating the regulatory genes for teicoplanin production in Actinoplanes teichomyceticus

The choice of vectors that can be conjugally transferred and maintained in A. teichomyceticus are extended and antibiotic producing properties and stability of the transconjugants have been examined.

Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

Since the cost and the quality of teicoplanin production depend mainly on the fermentation process, this work developed a robust and scalable fermentation process by using an industrial medium in which a complex composition can be modulated by the combined addition of suitable precursors.

Chemical tailoring of teicoplanin with site-selective reactions.

Chemical reactions that achieve site-selective alteration of teicoplanin may lead to analogues with attenuated or enhanced antibacterial properties, in particular against vancomycin- and teioplanin-resistant strains.

Characterization of the Post-Assembly Line Tailoring Processes in Teicoplanin Biosynthesis.

The generation and analysis of a set of A. teichomyceticus mutant strains that have been used to elucidate the sequence of reactions from the Tcp aglycone to mature Tcp are described and an updated order of post-assembly line tailoring processes in Tcp biosynthesis is proposed.


This review conceptualizes about the actinomycetes and its contribution to human health by playing a key role as bioactive secondary metabolites, such as enzymes, antibiotics and pigments, leading to

Progress in Understanding the Genetic Information and Biosynthetic Pathways behind Amycolatopsis Antibiotics, with Implications for the Continued Discovery of Novel Drugs

The efforts made in exploiting Amycolatopsis genome sequences for the discovery of novel natural products and biosynthetic pathways are summarized.

The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110

BackgroundActinoplanes sp. SE50/110 is known as the wild type producer of the alpha-glucosidase inhibitor acarbose, a potent drug used worldwide in the treatment of type-2 diabetes mellitus. As the

The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression

Analysis of the draft sequence of the genome of teicoplanin producer Actinoplanes teichomyceticus led to identification of several genes encoding AraC-family regulators that resemble AdpA, master regulator of transcription in Streptomyces, warranting its use as a tool to manipulate secondary metabolome of actinomycetes.



Production of teicoplanin by valine analogue-resistant mutant strains of Actinoplanes teichomyceticus

A strain improvement to increase the productivity of the major component, teicoplanin A2-2, was carried out and L-valine analogue (valine hydroxamate)-resistant mutants were derived.

Vancomycin, teicoplanin, and ramoplanin: Synthetic and mechanistic studies †

  • D. Boger
  • Medicine, Biology
    Medicinal research reviews
  • 2001
This work lays the foundation for ongoing structure–function studies on the antibiotics that may clarify or define their site and mechanism of action leading to the development of improved or reengineered antibiotics.

Production of the glycopeptide antibiotic A40926 by Nonomuraea sp. ATCC 39727: influence of medium composition in batch fermentation

It was found that low initial concentrations of phosphate and ammonium are beneficial for A40926 production and that productivity is not enhanced during glucose limitation, and the initiation of A 40926 production was not governed by residual ammonium and phosphate concentrations, although the level of these nutrients strongly influenced A409 26 production rates and final titers.

Teicoplanin Chemistry and Microbiology

Despite the increasing importance of glycopeptide resistance, teicoplanin has proved its clinical worth and continues to have important potential in the treatment of life-threatening Gram-positive sepsis.

Teicoplanin, a new antibiotic from Actinoplanes teichomyceticus nov. sp

The mechanism of action of teicoplanin is discussed in comparison with those of other inhibitors of cell wall biosynthesis, namely, vancomycin, ristocetin, and gardimycin.

Identification of a Deacetylase Involved in the Maturation of Teicoplanin

The chemical modification of natural glycopeptide scaffolds is proving a successful route to clinically useful compounds, although a potentially more attractive route to novel glycopePTides is through engineered biosynthesis.

The structural basis for induction of VanB resistance.

It is thought that lipid-containing analogues of vancomycin, like teicoplanin itself, circumvent resistance because the lipid chain changes the periplasmic distribution of the glycopeptide and, therefore, changes the biosynthetic step that it blocks.

Production of teicoplanin by Actinoplanes teichomyceticus in continuous fermentation

Production of the potent antibiotic teicoplanin by Actinoplanes teichomyceticus was studied in batch and in chemostat cultures and the derived kinetics almost exactly simulated the behavior of the cell recirculation reactor.

Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus.

The tcp gene cluster, devoted to teicoplanin biosynthesis in the actinomycete Actinoplanes teichomyceticus, was isolated and characterized and putative roles could be assigned for most of the tcp genes.

Teichomycins, new antibiotics from Actinoplanes teichomyceticus Nov. Sp. I. Description of the producer strain, fermentation studies and biological properties.

Studies of medium and fermentation conditions indicated that the highest antibiotic titers, ca 900 u/ml, are obtained in a medium containing 1% (w/v) glucose, 2% cotton seed meal, 1% malt extract, and 0.4% yeast extract.