Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies.


The main purpose of the present work was to evaluate if low laser level therapy (LLLT) can improve the effects of Biosilicate®/PLGA (BS/PLGA) composites on cell viability and bone consolidation using a tibial defects of rats. The composites were characterized by scanning electron microscope (SEM) and reflection Fourier transform infrared spectrometer (FTIR). For the in vitro study, fibroblast and osteoblast cells were seeded in the extract of the composites irradiated or not with LLLT (Ga-Al-As, 808nm, 10J/cm2) to assess cell viability after 24, 48 and 72h. For the in vivo study, 80 Wistar rats with tibial bone defects were distributed into 4 groups (BS; BS+LLLT; BS/PLGA and BS/PLGA+LLLT) and euthanized after 2 and 6weeks. Laser irradiation Ga-Al-As (808nm, 30J/cm2) in the rats was performed 3 times a week. The SEM and FTIR results revealed that PLGA were successfully inserted into BS and the microparticles degraded over time. The in vitro findings demonstrated higher fibroblast viability in both BS/PLGA groups after 24h and higher osteoblast viability in BS/PLGA+LLLT in all periods. As a conclusion, animals treated with BS/PLGA+LLLT demonstrated an improved material degradation and an increased amount of granulation tissue and newly formed bone.

DOI: 10.1016/j.jphotobiol.2017.06.002

Cite this paper

@article{Fernandes2017BiosilicatePLGAOE, title={Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies.}, author={Kelly Rosseti Fernandes and Angela Maria Paiva Magri and Hueliton Wilian Kido and Julia R Parisi and L Assis and Kristianne Porta Santos Fernandes and Raquel Agnelli Mesquita-Ferrari and Virginia Conceiç{\~a}o Amaro Martins and Ana Maria de Guzzi Plepis and Edgar Dutra Zanotto and Oscar Peitl and Ana Cl{\'a}udia Muniz Renn{\'o}}, journal={Journal of photochemistry and photobiology. B, Biology}, year={2017}, volume={173}, pages={258-265} }