Bioseparation of papain from Carica papaya latex by precipitation of papain-poly (vinyl sulfonate) complexes.

@article{Braia2013BioseparationOP,
  title={Bioseparation of papain from Carica papaya latex by precipitation of papain-poly (vinyl sulfonate) complexes.},
  author={Mauricio Javier Braia and M. Montserrat Real Ferrero and Mar{\'i}a Victoria Rocha and Dana Bel{\'e}n Loureiro and Gisela Tub{\'i}o and Diana Romanini},
  journal={Protein expression and purification},
  year={2013},
  volume={91 1},
  pages={
          91-5
        }
}
The formation of insoluble complexes between enzymes and polyelectrolytes is a suitable technique for isolating these biomolecules from natural sources, because it is a simple and rapid technique that allows the concentration of the protein. This technique can be used in most purification protocols at the beginning of the downstream process. The aim of this investigation is to isolate papain from Carica papaya latex by precipitation of insoluble complexes between this enzyme and poly (vinyl… CONTINUE READING
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