Biosensor with L-phenylalanine Dehydrogenase and Diaphorase Immobilized on Nanocomposite Electrode for the Determination of L-phenylalanine in Urine

Abstract

Bienzymatic amperometric biosensor consisting of L-phenylalanine dehydrogenase and diaphorase for the determination of L-phenylalanine has been developed. Enzymes were immobilized between chitosan layers onto the surface of planar nanocomposite electrodes based on multi-walled carbon nanotubes. Wide linear concentration range was obtained for 12–1250 μM of… (More)

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