Biosensor recognition of thyroid-disrupting chemicals using transport proteins.

@article{Marchesini2006BiosensorRO,
  title={Biosensor recognition of thyroid-disrupting chemicals using transport proteins.},
  author={Gerardo R Marchesini and Eline P. Meulenberg and Willem Haasnoot and Mineyuki Mizuguchi and Hubertus Irth},
  journal={Analytical chemistry},
  year={2006},
  volume={78 4},
  pages={1107-14}
}
Novel surface plasmon resonance-based biosensor assays for the bioeffect-related screening of chemicals with thyroid-disrupting activity are described. Two thyroid transport proteins (TPs), thyroxine binding globulin (TBG) and recombinant transthyretin (rTTR), were applied in an inhibition assay format in a Biacore 3000 using CM5 biosensor chips coated with l-thyroxine (T4), the main hormone of the thyroid system. Assay conditions were optimized for the natural thyroid hormones, and known… CONTINUE READING