Biosensor based on Langmuir–Blodgett films of poly(3-hexyl thiophene) for detection of galactose in human blood

Abstract

An amperometric biosensor was developed to estimate galactose in human blood serum. Monolayers of poly(3-hexyl thiophene) were placed on glass plates coated with indium tin oxide formed by dispensing a mixed solution of stearic acid in chloroform on to a water sub-phase. Galactose oxidase was mixed with poly(3-hexyl thiophene)/stearic acid in chloroform and dispensed on to the air-water interface of Langmuir–Blodgett trough. These monolayers were transferred on to glass plates which were used as working electrodes with platinum as a reference electrode. The amperometric galactose biosensor thus fabricated had a linear response from 0.05 to 0.5 g galactose l−1 in blood serum. The normal level in blood is < 0.05 g galactose l−1 in adults and 0–0.2 g galactose l−1 in infants. In case of galactosemia, this increases to above 0.2 g galactose l−1 in infants.

DOI: 10.1023/B:BILE.0000023023.19651.01

3 Figures and Tables

Statistics

050100150'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

62 Citations

Semantic Scholar estimates that this publication has 62 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Sharma2004BiosensorBO, title={Biosensor based on Langmuir–Blodgett films of poly(3-hexyl thiophene) for detection of galactose in human blood}, author={Sandeep K Sharma and Rahul Singhal and Bansi Dhar Malhotra and Neeta Sehgal and Ashok Kumar}, journal={Biotechnology Letters}, year={2004}, volume={26}, pages={645-647} }