An amperometric biosensor was developed to estimate galactose in human blood serum. Monolayers of poly(3-hexyl thiophene) were placed on glass plates coated with indium tin oxide formed by dispensing a mixed solution of stearic acid in chloroform on to a water sub-phase. Galactose oxidase was mixed with poly(3-hexyl thiophene)/stearic acid in chloroform and… (More)
Fig. 2. Amperometric response of galactose oxidase immobilized in poly(3-hexyl thiophene) and stearic acid Langmuir–Blodgett films at 0.2 g galactose l−1 in 0.1 M phosphate buffer, pH 7.4 as a function of number of measurements using same electrode.