Bioreactor expansion of human bone marrow: comparison of unprocessed, density-separated, and CD34-enriched cells.

@article{Koller1995BioreactorEO,
  title={Bioreactor expansion of human bone marrow: comparison of unprocessed, density-separated, and CD34-enriched cells.},
  author={Manfred R. Koller and I Manchel and B S Newsom and M A Palsson and Bernhard O. Palsson},
  journal={Journal of hematotherapy},
  year={1995},
  volume={4 3},
  pages={159-69}
}
Scale-up of human hematopoietic cultures was previously described in continuously perfused systems with bone marrow mononuclear cells (BMMNC), yielding expansion of both progenitors and long-term culture-initiating cells (LTC-IC). We report here on the use of these systems for expansion of unprocessed whole BM cells (WBMC) and CD34-enriched cells. Density separation recovered 84% of CFU-GM and 65% of LTC-IC from WBMC. Subsequent CD34 selection recovered 17% of CFU-GM and 48% of LTC-IC from the… CONTINUE READING
16 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…