Biopsy and sex determination by PCR of IVF bovine embryos.

  title={Biopsy and sex determination by PCR of IVF bovine embryos.},
  author={Zolt{\'a}n Mach{\'a}ty and A P{\'a}ldi and T Cs{\'a}ki and Zsuzsa R{\'e}ka Varga and Ildik{\'o} Kiss and Z B{\'a}r{\'a}ndi and G{\'a}bor Vajta},
  journal={Journal of reproduction and fertility},
  volume={98 2},
Sex of early bovine embryos was determined by polymerase chain reaction (PCR) using a single blastomere removed at the 16-32 cell stage. Embryos were produced in vitro and biopsied on the fifth day after in vitro fertilization. Biopsied embryos were cultured on a cumulus cell monolayer until embryo transfer. For the PCR, one pair of bovine-specific and one… CONTINUE READING