Bioprospecting for Antibacterial Drugs: a Multidisciplinary Perspective on Natural Product Source Material, Bioassay Selection and Avoidable Pitfalls

  title={Bioprospecting for Antibacterial Drugs: a Multidisciplinary Perspective on Natural Product Source Material, Bioassay Selection and Avoidable Pitfalls},
  author={Tim Cushnie and Benjamart Cushnie and Javier Echeverr{\'i}a and Winita Fowsantear and Sutthiwan Thammawat and J. Dodgson and S. Law and Simon M. Clow},
  journal={Pharmaceutical Research},
Bioprospecting is the exploration, extraction and screening of biological material and sometimes indigenous knowledge to discover and develop new drugs and other products. Most antibiotics in current clinical use (eg. β-lactams, aminoglycosides, tetracyclines, macrolides) were discovered using this approach, and there are strong arguments to reprioritize bioprospecting over other strategies in the search for new antibacterial drugs. Academic institutions should be well positioned to lead the… Expand
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  • M. Moloney
  • Biology, Medicine
  • Trends in pharmacological sciences
  • 2016
This overview examines the current situation, highlights new natural product systems which have been found, together with re-examination of some old ones, and new technologies for their identification. Expand
How to translate a bioassay into a screening assay for natural products: general considerations and implementation of antimicrobial screens.
Some aspects which are of significant concern when screening natural products in a microtiter plate-based format are discussed, being partly applicable to other assay formats as well, such as validation parameters, layouts for assay protocols, and common interferences caused by natural products samples, as well as various troubleshooting strategies. Expand
From Worms to Drug Candidate: The Story of Odilorhabdins, a New Class of Antimicrobial Agents
Odilorhabdins (ODLs), a novel antibacterial class, were identified from this campaign and inhibit bacterial translation by binding to the small ribosomal subunit at a site not exploited by current antibiotics. Expand
Alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities.
The synthetic isoquinoline alkaloid virstatin, for example, inhibits the transcriptional regulator ToxT in Vibrio cholerae, preventing expression of cholera toxin and fimbriae and conferring in vivo protection against intestinal colonisation. Expand
Endophytes as sources of antibiotics.
The present review article compiles recent (2006-2016) literature to provide an update on endophyte research aimed at finding metabolites with antibiotic activities to show that endophytes that produce bioactive compounds have been collected globally. Expand
Anti-infective potential of natural products: how to develop a stronger in vitro 'proof-of-concept'.
This review provides a number of recommendations that will help to define a more sound 'proof-of-concept' for antibacterial, antifungal, antiviral and antiparasitic potential in natural products. Expand
New environmentally-friendly antimicrobials and biocides from Andean and Mexican biodiversity.
Phytochemicals from a number of families of plants and fungi from the southern Andes and from Mexico have now been evaluated and offer new approaches for pest management by providing new sources of biologically active natural products with biodegradability, low mammalian toxicity and environmentally-friendly qualities. Expand
Screening methods to determine antibacterial activity of natural products
Evaluating technical variants used in screening methods to determine antibacterial activity of natural products found bioautographic and diffusion techniques were found to have similar sensitivity; however the latter technique provided more suitable conditions for the microbial growth. Expand
Phytochemicals for bacterial resistance--strengths, weaknesses and opportunities.
This review covers some of the opportunities which currently exist to exploit plants for their natural products as templates for new antibacterial substances. This is a timely exercise given theExpand
New approaches to antimicrobial discovery
  • K. Lewis
  • Biology, Medicine
  • Biochemical pharmacology
  • 2017
Graphical abstract Figure. No Caption available. Abstract The spread of resistant organisms is producing a human health crisis, as we are witnessing the emergence of pathogens resistant to allExpand