Bioprospecting for Antibacterial Drugs: a Multidisciplinary Perspective on Natural Product Source Material, Bioassay Selection and Avoidable Pitfalls

  title={Bioprospecting for Antibacterial Drugs: a Multidisciplinary Perspective on Natural Product Source Material, Bioassay Selection and Avoidable Pitfalls},
  author={Tim Cushnie and Benjamart Cushnie and Javier Echeverr{\'i}a and Winita Fowsantear and Sutthiwan Thammawat and Jolyon L. A. Dodgson and Samantha Law and Simon M. Clow},
  journal={Pharmaceutical Research},
Bioprospecting is the exploration, extraction and screening of biological material and sometimes indigenous knowledge to discover and develop new drugs and other products. Most antibiotics in current clinical use (eg. β-lactams, aminoglycosides, tetracyclines, macrolides) were discovered using this approach, and there are strong arguments to reprioritize bioprospecting over other strategies in the search for new antibacterial drugs. Academic institutions should be well positioned to lead the… 
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Natural Products as a Source for Novel Antibiotics.
  • M. Moloney
  • Biology
    Trends in pharmacological sciences
  • 2016
How to translate a bioassay into a screening assay for natural products: general considerations and implementation of antimicrobial screens.
Some aspects which are of significant concern when screening natural products in a microtiter plate-based format are discussed, being partly applicable to other assay formats as well, such as validation parameters, layouts for assay protocols, and common interferences caused by natural products samples, as well as various troubleshooting strategies.
From Worms to Drug Candidate: The Story of Odilorhabdins, a New Class of Antimicrobial Agents
Odilorhabdins (ODLs), a novel antibacterial class, were identified from this campaign and inhibit bacterial translation by binding to the small ribosomal subunit at a site not exploited by current antibiotics.
Screening methods to determine antibacterial activity of natural products
Evaluating technical variants used in screening methods to determine antibacterial activity of natural products found bioautographic and diffusion techniques were found to have similar sensitivity; however the latter technique provided more suitable conditions for the microbial growth.
Phytochemicals for bacterial resistance--strengths, weaknesses and opportunities.
This review covers some of the opportunities which currently exist to exploit plants for their natural products as templates for new antibacterial substances. This is a timely exercise given the
Rare actinomycetes: a potential storehouse for novel antibiotics
It is argued that the likelihood of discovering a new compound having a novel chemical structure can be increased with intensive efforts in isolating and screening rare genera of microorganisms, particularly actinomycetes.
Beyond Traditional Antimicrobials: A Caenorhabditis elegans Model for Discovery of Novel Anti-infectives
An overview on use of the C. elegans model for identification of novel anti-infectives, particularly those that target bacterial virulence or host defense to eradicate infections, a mechanism distinct from the action of conventional antibiotics.