Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2‐based 3‐hydroxypropionate production

  title={Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2‐based 3‐hydroxypropionate production},
  author={Aaron B Hawkins and H. Lian and Benjamin M. Zeldes and A. Loder and G. L. Lipscomb and G. Schut and Matthew W. Keller and M. Adams and R. Kelly},
  journal={Biotechnology and Bioengineering},
  • Aaron B Hawkins, H. Lian, +6 authors R. Kelly
  • Published 2015
  • Biology, Medicine
  • Biotechnology and Bioengineering
  • Metabolically engineered strains of the hyperthermophile Pyrococcus furiosus (Topt 95–100°C), designed to produce 3‐hydroxypropionate (3HP) from maltose and CO2 using enzymes from the Metallosphaera sedula (Topt 73°C) carbon fixation cycle, were examined with respect to the impact of heterologous gene expression on metabolic activity, fitness at optimal and sub‐optimal temperatures, gas‐liquid mass transfer in gas‐intensive bioreactors, and potential bottlenecks arising from product formation… CONTINUE READING
    20 Citations
    A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1
    • Highly Influenced
    Biotechnology of extremely thermophilic archaea.
    • 17
    • PDF


    Engineering a Hyperthermophilic Archaeon for Temperature-Dependent Product Formation
    • 49
    • PDF
    Microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus exposed to gamma irradiation
    • 63
    • PDF
    Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C
    • 500
    Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide
    • 97
    • PDF