Bioprocess applications of a Sindbis virus-based temperature-inducible expression system.

@article{Boorsma2002BioprocessAO,
  title={Bioprocess applications of a Sindbis virus-based temperature-inducible expression system.},
  author={Marco Boorsma and S Hoenke and Alma Marrero and Rainer Fischer and J. E. Bailey and Wolfgang A. Renner and Marlin F Bachmann},
  journal={Biotechnology and bioengineering},
  year={2002},
  volume={79 6},
  pages={602-9}
}
The production and study of toxic proteins requires inducible expression systems with low basal level expression and high inducibility. Here, we describe bioprocess applications of the pCytTS temperature-regulatable Sindbis virus replicon-based expression system. We used green fluorescent protein as a marker protein to optimize the selection of stable transfected clones with increased expression levels. Using the optimized protocol, clones were constructed that produced the growth-inhibiting… CONTINUE READING

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