Biophysics of the green fluorescent protein.

  title={Biophysics of the green fluorescent protein.},
  author={Franklyn G. Prendergast},
  journal={Methods in cell biology},
It is almost certainly a truism that interpretation of the fluorescence of a protein matrix-embedded chromophore in terms of the physicochemical character of its environment requires that the tertiary structure of the protein be known to high resolution. This reality derives from the complexity of the photophysics of most fluorescent molecules, complexity that reveals the imperfections of available theory. The accuracy of these dicta is highlighted by the biophysical properties of the green… CONTINUE READING

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