Biophysical studies on agents affecting the state of membrane lipids: Biochemical and pharmacological implications

  title={Biophysical studies on agents affecting the state of membrane lipids: Biochemical and pharmacological implications},
  author={Giorgio Lenaz and Giovanna Curatola and Laura Mazzanti and Giovanna Parenti-Castelli},
  journal={Molecular and Cellular Biochemistry},
SummaryThe phospholipid requirement of membrane-bound enzymes may depend on several reasons. In our laboratory we have investigated lipids (1) as a bidimensional medium required for the movement of Coenzyme Q, a lipid-soluble cofactor of the mitochondrial respiratory chain, and (2) as a hydrophobic environment necessary to impose the proper conformation to membrane-bound enzymic proteins.We have found that Coenzyme Q, once reduced by NADH dehydrogenase, must cross the inner mitochondrial… 

Temperature dependence of the fluorescence of pyrene labeled crab nerve membranes

The excimer-to-monomer fluorescence intensity ratio of pyrene is a fluidity parameter which displayed the following features with temperatures: a temperature-dependent increase of fluidity, three breaks in the physiological medium, and the relationship of these findings to nerve function.

Inhibition of bioenergetics alters intracellular calcium, membrane composition, and fluidity in a neuronal cell line

Results show that depletion of cellular ATP results in inhibition of the ER Ca2+-pump, loss of AA from membranes, and decreased membrane fluidity, and it is proposed that impaired bioenergetics can increase intracellularCa2+ as a result of Ca2+, and thereby activate Ca2-dependent phospholipases causing membrane effects.

The mapping of three subfractions of endoplasmic reticulum membranes isolated from L-929 cells by the use of spin probes

The results show that each membrane subfraction is characterized by an intrinsic series of thermoinduced structural transitions, which, in combination with an estimation of microviscosity, yields a ‘portrait’ of the structural state of the membrane lipids.

Kinetic Regulation of the Mitochondrial Glycerol-3-phosphate Dehydrogenase by the External NADH Dehydrogenase in Saccharomyces cerevisiae *

In the yeast Saccharomyces cerevisiae, the two most important systems for conveying excess cytosolic NADH to the mitochondrial respiratory chain are external NADH dehydrogenase (Nde1p/Nde2p) and the

A pressure-reversible cellular mechanism of general anesthetics capable of altering a possible mechanism for consciousness

The common mechanism of anesthetics presented here can operate along with the known specific actions of different anestheses, and can alter the conformation of the integral of the units of internal sensations that maintain consciousness.

Sterols Bioconversions in Systems with an Organic Phase

It was concluded that for this reaction, properties such as interfacial tensions, the molecule chain-length and presence of double bonds may influence the outcome of the enzyme activity.

Cholestatriene and ergostatetraene as in vivo and in vitro membrane and lipoprotein probes.

Results are discussed in terms of a thermotropic reordering of cholesterol clusters existing in the erythrocyte membrane and of the cholesteryl ester core present within the low density lipoprotein particle.

Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications

It is concluded that variations in the thermodynamic state (here π and T, in general pH, membrane potential V, etc also) of lipid membranes are capable of controlling fluorescence intensity.

Propagation of 2D pressure pulses in lipid monolayers and its possible implications for biology.

The role of interface density pulses in biology is discussed, in particular, in the context of communicating localized alterations in protein function (signaling) and nerve pulse propagation.



The interaction of glutamate dehydrogenase and malate dehydrogenase with phospholipid membranes.

  • G. Dodd
  • Biology, Chemistry
    European journal of biochemistry
  • 1973
NMR studies indicated that, as with the related enzyme 3-hydroxybutyrate dehydrogenase, both the polar choline head group and the hydrocarbon chains are involved in the binding of the lipid to the protein.