Biophysical studies of engineered mutant proteins based on calbindin D9k modified in the pseudo EF-hand.

@article{Johansson1990BiophysicalSO,
  title={Biophysical studies of engineered mutant proteins based on calbindin D9k modified in the pseudo EF-hand.},
  author={Cecilia Johansson and Peter Brodin and Thomas Grundstr{\"o}m and Eva Thulin and Sture Fors{\'e}n and Torbjorn Drakenberg},
  journal={European journal of biochemistry},
  year={1990},
  volume={187 2},
  pages={455-60}
}
The genes for four mutant proteins from calbindin D9k, all with mutations in the N-terminal Ca2+-binding domain (pseudo EF-hand) have been synthesized and expressed in Escherichia coli. The purification scheme has been modified to minimize the formation of deamidated proteins. The set of modifications in the pseudo EF-hand is an attempt to turn this site into a structure resembling an archetypal EF-hand, with its characteristic 113Cd-NMR shift (-80 to -110 ppm) and high calcium-binding… CONTINUE READING

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