Biophysical response to pulsed laser microbeam-induced cell lysis and molecular delivery.

  title={Biophysical response to pulsed laser microbeam-induced cell lysis and molecular delivery.},
  author={Amy N Hellman and K. Raghava Rau and Helen H Yoon and Vasan Venugopalan},
  journal={Journal of biophotonics},
  volume={1 1},
Cell lysis and molecular delivery in confluent monolayers of PtK(2) cells are achieved by the delivery of 6 ns, lambda = 532 nm laser pulses via a 40x, 0.8 NA microscope objective. With increasing distance from the point of laser focus we find regions of (a) immediate cell lysis; (b) necrotic cells that detach during the fluorescence assays; (c) permeabilized cells sufficient to facilitate the uptake of small (3 kDa) FITC-conjugated Dextran molecules in viable cells; and (d) unaffected, viable… CONTINUE READING
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Investigation of laser-induced cell lysis using time-resolved imaging

  • K. R. Rau, A. G. Guerra, A. Vogel, V. Venugopalan
  • Applied Physics Letters,
  • 2004
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