Corpus ID: 40220328

Biomimetic recognition and immunochemical assay of ligand binding to collagen.

  title={Biomimetic recognition and immunochemical assay of ligand binding to collagen.},
  author={J. Vaidyanathan and K. Chinnaswamy and T. Vaidyanathan},
  journal={The journal of adhesive dentistry},
  volume={5 1},
PURPOSE The objectives of this investigation were to analyze the adhesive interactions between type 1 collagen and two hydrophilic monomer primers (ligands) for dentin bonding through computer modeling and a novel immunochemical binding assay method. MATERIALS AND METHODS The hydrophilic monomers studied included 2-hydroxyethyl methacrylate (HEMA) and 2-acryloyloxyethyl phosphate (PA). For computer modeling studies, a triple helical model structure of collagen fibril extracted from a public… Expand
Evaluation of intermolecular interactions of self-etch dentin adhesive primer molecules with type 1 collagen: computer modeling and in vitro binding analysis.
It is suggested that the ability of self-etch dentin primer systems to bond effectively to dentin is not limited to the interaction of the primer with the hydroxyapatite of dentin, but also due to the ability to prime dentin efficiently through intermolecular interactions between the primer and its calcium salt with the collagen matrix. Expand
Computer simulated screening of dentin bonding primer monomers through analysis of their chemical functions and their spatial 3D alignment.
The results elegantly demonstrate that an indirect approach used to identify pharmacaphore models from adhesive ligands without a target may be a simple and viable approach to assess their intermolecular interactions with an intended target. Expand
Recent advances in the theory and mechanism of adhesive resin bonding to dentin: a critical review.
This review analyzes current theoretical and mechanism-related issues of interest in adhesive resin bonding to dentin, and outlines the continuing problems that need to be overcome in the future. Expand
Water permeability, hybrid layer long-term integrity and reaction mechanism of a two-step adhesive system.
The results showed the hydrophilicity of the adhesive, which formed an IPN-like hybrid layer that conserved adequate impermeability over a 1 year period, is capable of both dissolving calcium ions and binding to apatite surfaces. Expand
In vitro regulation of single collagen fibril length by buffer compositions and temperature.
Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer, and at the same temperature, the average lengths of single fibril lengths differed significantly between PBS and SP buffers. Expand
The effect of cationic polymer treatment on dye staining and on the adhesion of charged particles to dentin.
Dentin treatment with a cationic polymer inhibits the staining of dentin by cationo dye and adherence of cationi beads and the pattern of adherence is altered by catedic polymer application. Expand
Photocrosslinkable Gelatin Hydrogels Modulate the Production of the Major Pro-inflammatory Cytokine, TNF-α, by Human Mononuclear Cells
This work investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform and found tumor necrosis factor-α expression was suppressed in GelMA culture conditions. Expand
Effect of anions on type I collagen fibrillogenesis in aqueous solution
  • J. Xing, L. Yang, Y. Li
  • Chemistry
  • 2011 International Symposium on Water Resource and Environmental Protection
  • 2011
Collagen is the most abundant protein in the human body. Fibrillogenesis is one of important aspect of collagen. In vitro reconstitution of collagen fibrils is triggered simply by warming,Expand
Development of biomimetic platforms to investigate the influence of the extra-cellular environment on immunological responses
The development and characterisation of tissue-engineered platforms for immune cell culture which incorporate the ECM and shear flow are described to apply these platforms for the study of the effect of the extra-cellular environment on dendritic cells and their interactions with T cells in the context of immunological stimulation. Expand
Water Concentration in Self-etching Primers Affects their Aggressiveness and Bonding Efficacy to Dentin
Water is required to ionize acid resin monomers for demineralization of tooth substrates and a balance must be achieved to provide sufficient water for adequate ionization of the acidic monomers, without lowering the resin concentration too much, to optimize their bonding efficacy to dentin. Expand