Biomedical three–dimensional holographic microimaging at visible, ultraviolet and X–ray wavelengths

  title={Biomedical three–dimensional holographic microimaging at visible, ultraviolet and X–ray wavelengths},
  author={Keith Boyer and Johndale C. Solem and James W. Longworth and Alex B. Borisov and Charles K. Rhodes},
  journal={Nature Medicine},
A new imaging technology is under advanced development that has several key advantages over conventional forms of microimaging performed with standard light microscopes, confocal light microscopes, and electron microscopes. The image created by this microscope possesses several unique features: It is intrinsically three-dimensional; it can be formed with very high contrast, a characteristic of the phase-sensitive nature of the recording; the information contained in the image is obtained in a… 
Digital holography applied to microscopy
We report on new developments of a technique called Digital Holographic Microscopy (DHM), for the numerical reconstruction of digital holograms taken in microscopy, which allows simultaneous
Holographic Printing of White-Light Viewable Holograms and Stereograms
The holographic printing followed the two main approaches in this area: i) computation of the holographic fringes by numerical simulation of the interference and encoding the resulting pattern onto a suitable medium for further optical display.
Ultrabright multikilovolt coherent tunable x-ray source at λ ~ 2.71–2.93 Å
Detailed molecular structural information of the living state is of enormous significance to the medical and biological communities. Since hydrated biologically active structures are small delicate
Cells and tissue imaging with digital holographic microscopy
Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.
Optical tomography at the microscopic scale by means of a numerical low-coherence holographic technique
We describe here a numerical application of the optical low- coherence holographic microscopy (OLCHM) technique. A low coherent source illuminates an object and the backscattered light interfere with
Digital recording and numerical reconstruction of holograms: an optical diagnostic for combustion.
The principles of Fourier optics are applied to develop two numerical methods for hologram reconstruction, and it is shown that both methods provide an accurate reconstruction of the phase image associated with a flame.
Basic principles and applications of digital holographic microscopy
This Chapter presents the analysis of the capabilities provided by DHM when studying a fixed swine sperm biosample and overviews the main application fields of DHM.
Enhanced robustness digital holographic microscopy for demanding environment of space biology
We describe an optimized digital holographic microscopy system (DHM) suitable for high-resolution visualization of living cells under conditions of altered macroscopic mechanical forces such as those
Development of phase-contrast X-ray imaging techniques and potential medical applications.
  • Shu‐Ang Zhou, A. Brahme
  • Physics
    Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics
  • 2008


Fourier-transform holographic microscope.
A holographic microscope with a spatial resolution approaching the diffraction limit and two unique capabilities that can collect three-dimensional information in a single pulse of light, avoiding specimen damage and bleaching is described.
Holography at x-ray wavelengths
We discuss alternative holographic techniques for imaging microscopic structures with a short-pulse, high intensity, high-quantum-energy laser. We find that Fresnel transform holography using a
X-Ray Biomicroholography
We discuss alternative techniques for microholography of biological specimens including requirements and constraints on the optical elements and recording media. We derive spatial and temporal
Microholography of Living Organisms
By using intense pulsed coherent x-ray sources that are currently under development, it will be possible to obtain magnified three-dimensional images of elementary biological structures in the living
X-Ray Holography of Microobjects
Possibilities of x-ray holography with the use of synchrotron radiation of modern electron storage rings are investigated. The required exposure time of a hologram and also that required of detected
Ultrahigh power compression for X-ray amplification: multiphoton cluster excitation combined with nonlinear channelled propagation
The ability to apply power densities controllably at or above vigorous thermonuclear levels (>1019 W cm-3) in materials is the basic issue for achieving efficient amplification of X-rays. Recent
Rethinking cell structure.
  • S. Penman
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1995
Embedment-free electron microscopy gives clear, high-contrast micrographs of cell structure when combined with removal of obscuring material such as soluble proteins.
Optical Information Processing
This is the second volume on "Optical Information Processing" within the scope of the US-USSR Science Cooperation Program co- sponsored by the US National Science Foundation and the USSR Acad- emy of