Biomass measurement of methane forming bacteria in environmental samples.

@article{Martz1983BiomassMO,
  title={Biomass measurement of methane forming bacteria in environmental samples.},
  author={R F Martz and Daniel I. Sebacher and D. Carl White},
  journal={Journal of microbiological methods},
  year={1983},
  volume={1},
  pages={53-61}
}
Methane-forming bacteria contain unusual phytanylglycerol ether phospholipids which can be extracted from the bacteria in sediments and assayed quantitatively by high performance liquid chromatography (HPLC). In this procedure the lipids were extracted, the phospholipids recovered, hydrolyzed, purified by thin layer chromatography, derivatized and assayed by HPLC. Ether lipids were recovered quantitatively from Methanobacterium thermoautotrophicum and sediments at levels as low as 8 x 10(-14… CONTINUE READING

From This Paper

Topics from this paper.