Bioluminescent assays for high-throughput screening.

@article{Fan2007BioluminescentAF,
  title={Bioluminescent assays for high-throughput screening.},
  author={Frank Fan and Keith V. Wood},
  journal={Assay and drug development technologies},
  year={2007},
  volume={5 1},
  pages={
          127-36
        }
}
  • F. Fan, K. Wood
  • Published 13 March 2007
  • Biology
  • Assay and drug development technologies
In the development of high throughput screening (HTS) as a central paradigm of drug discovery, fluorescence has generally been adopted as the favored methodology. [] Key Result In genetic reporter applications, modifications to the luciferase genes have improved assay sensitivity by substantially increasing expression efficiency and enhanced response dynamics by reducing expression lifetime.

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Bioluminescent assays for ADMET
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Three bioluminescent ADMET assay types that use firefly luciferase to measure properties of drugs and other xenobiotics which affect their absorption, distribution, metabolism, elimination and toxicity are described.
High Throughput Screening (HTS) Techniques: Applications in Chemical Biology
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HTS systems can expand the scope of an experimental hypothesis to address questions of chemical biology, be they at the level of an isolated enzymatic activity or that of a complex cellular phenotype, as well as define compound class/gene family selectivity and any other relationship between chemical structure and bioactivity.
Update on in vitro cytotoxicity assays for drug development
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This work focuses on three main classes of assays that are at present the most popular, useful, and practical for HTS drug discovery efforts and suggests multi-parametric methods using both viability and cytotoxicity markers may mitigate the inherent shortcomings of single parameter measures.
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