Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions

@article{Pfleger2006BioluminescenceRE,
  title={Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions},
  author={Kevin Donald George Pfleger and Ruth M. Seeber and Karin Ann Eidne},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={337-345}
}
A substantial range of protein-protein interactions can be readily monitored in real time using bioluminescence resonance energy transfer (BRET). The procedure involves heterologous coexpression of fusion proteins, which link proteins of interest to a bioluminescent donor enzyme or acceptor fluorophore. Energy transfer between these proteins is then detected. This protocol encompasses BRET1, BRET2 and the recently described eBRET, including selection of the donor, acceptor and substrate… CONTINUE READING
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