Bioluminescence detection system of mutagen using firefly luciferase genes introduced in Escherichia coli lysogenic strain.


A rapid and convenient microbial sensing system for mutagens was developed based upon the induction of prophage from Escherichia coli lysogenic strain and bioluminescence. The system consisted of lysogenic E. coli encoding firefly luciferase genes and a photodetection system. Measurement of mutagen mitomycin C was achieved by measuring the luminescence… (More)


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