Biology and Taxonomy of crAss-like Bacteriophages, the Most Abundant Virus in the Human Gut.

@article{Guerin2018BiologyAT,
  title={Biology and Taxonomy of crAss-like Bacteriophages, the Most Abundant Virus in the Human Gut.},
  author={Emma Guerin and Andrey Nikolaevich Shkoporov and Stephen R. Stockdale and Adam G. Clooney and Feargal J. Ryan and Thomas D S Sutton and Lorraine A. Draper and Enrique Gonz{\'a}lez-Tortuero and Reynolds Paul Ross and Colin Hill},
  journal={Cell host \& microbe},
  year={2018},
  volume={24 5},
  pages={
          653-664.e6
        }
}
Unique genomic features of crAss-like phages, the dominant component of the human gut virome
TLDR
Comparative genomic analysis of the expanded assemblage of crAss-like phages reveals several unusual features of genome architecture and expression as well as phage biology that were not apparent from the previous crAssphage analyses.
Discovery, diversity and functional associations of crAss-like phages in human gut metagenomes from four Dutch cohorts
TLDR
Gut metagenomic data collected from more than 2000 individuals from the Netherlands discovered 125 novel species-level and 32 novel genus-level clusters of crAss-like phages, all belonging to five previously recognized groups associated with the human gut.
Analysis of metagenome-assembled viral genomes from the human gut reveals diverse putative CrAss-like phages with unique genomic features
TLDR
Comparative genomic analysis of the expanded assemblage of crAss-like phages reveals aspects of genome architecture and expression as well as phage biology that were not apparent from the previous work on phage genomics.
Isolation and characterisation of ΦcrAss002, a crAss-like phage from the human gut that infects Bacteroides xylanisolvens
TLDR
The isolation and characterisation of ΦcrAss002 is reported, the first representative of the proposed Alphacrassvirinae sub-family of crAss-like phages, which cannot form plaques or spots on bacterial lawns but can co-exist with its host, Bacteroides xylanisolvens, at very high levels in liquid culture without impacting on bacterial numbers.
Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
TLDR
The whole genome shotgun project of the Mexican-crAssphage is available at DDBJ/ENA/GenBank under the GenBank MK069403 and provides valuable information for the experimental characterization of the most abundant human gut bacteriophage.
Evolutionary Study of the Crassphage Virus at Gene Level
TLDR
This study provides an in-depth picture of the evolution of different genes in crAss-like viruses, and confirms that the genes encoding capsid proteins were the most ubiquitous, despite their overall low sequence conservation.
Metagenomic compendium of 189,680 DNA viruses from the human gut microbiome
TLDR
The Metagenomic Gut Virus catalogue improves detection of viruses in stool metagenomes and accounts for nearly 40% of CRISPR spacers found in human gut Bacteria and Archaea and 459,375 viral protein clusters to explore the functional potential of the gut virome.
Acquisition, transmission and strain diversity of human gut-colonizing crAss-like phages
TLDR
It is found that crAss-like phages are rarely detected at birth but are increasingly prevalent in the infant microbiome after one month of life, and that strain diversity in infants increases with time.
Bacteroides thetaiotaomicron-infecting bacteriophage isolates inform sequence-based host range predictions
TLDR
A detailed view of the gut’s “viral dark matter” and a framework for future efforts to further integrate isolation- and sequencing-focused efforts to understand gut-resident phages are provided.
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