Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

@article{Takahashi2010BiologicalPO,
  title={Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.},
  author={Hitoshi Takahashi and Daisuke Akazawa and Takanobu Kato and Tomoko Date and Masayuki Shirakura and Noriko Nakamura and Hidenori Mochizuki and Keiko Tanaka-Kaneko and Tetsutaro Sata and Yasuhito Tanaka and Masashi Mizokami and Tetsuro Suzuki and Takaji Wakita},
  journal={Biochemical and biophysical research communications},
  year={2010},
  volume={395 4},
  pages={565-71}
}
To establish a simple system for purification of recombinant infectious hepatitis C virus (HCV) particles, we designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutation at the potential N-glycosylation site (E2N151K) leads to efficient production of the chimeric virus. This finding suggests the involvement of glycosylation at Asn within the… CONTINUE READING
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