Biological function and site II Ca2+-induced opening of the regulatory domain of skeletal troponin C are impaired by invariant site I or II Glu mutations.

@article{Pearlstone2000BiologicalFA,
  title={Biological function and site II Ca2+-induced opening of the regulatory domain of skeletal troponin C are impaired by invariant site I or II Glu mutations.},
  author={Joyce R. Pearlstone and Murali Chandra and Martha M. Sorenson and Lawrence B. Smillie},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 45},
  pages={35106-15}
}
To investigate the roles of site I and II invariant Glu residues 41 and 77 in the functional properties and calcium-induced structural opening of skeletal muscle troponin C (TnC) regulatory domain, we have replaced them by Ala in intact F29W TnC and in wild-type and F29W N domains (TnC residues 1-90). Reconstitution of intact E41A/F29W and E77A/F29W mutants into TnC-depleted muscle skinned fibers showed that Ca(2+)-induced tension is greatly reduced compared with the F29W control. Circular… CONTINUE READING

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