Biological electron energy loss spectroscopy in the field-emission scanning transmission electron microscope.

@article{Leapman1994BiologicalEE,
  title={Biological electron energy loss spectroscopy in the field-emission scanning transmission electron microscope.},
  author={Richard Leapman and Su qin Sun and John Alan Hunt and S. Brian Andrews},
  journal={Scanning microscopy. Supplement},
  year={1994},
  volume={8},
  pages={245-58; discussion 258-9}
}
The dedicated scanning transmission electron microscope (STEM) combined with parallel electron energy loss spectroscopy (EELS) provides a very sensitive means of detecting specific elements in small structures. EELS is more sensitive than optimized energy-dispersive X-ray spectroscopy by a factor of about three for calcium. Measurement of such low concentrations requires special processing methods such as difference-acquisition techniques and multiple least squares procedures for fitting… CONTINUE READING