Biological effects of helium-neon (He-Ne) laser irradiation on acrosome reaction in bull sperm cells.

@article{OcaaQuero1997BiologicalEO,
  title={Biological effects of helium-neon (He-Ne) laser irradiation on acrosome reaction in bull sperm cells.},
  author={J M Oca{\~n}a-Quero and Rafael Jes{\'u}s G{\'o}mez-Villamandos and M. Moreno-Mill{\'a}n and J M Santisteban-Valenzuela},
  journal={Journal of photochemistry and photobiology. B, Biology},
  year={1997},
  volume={40 3},
  pages={
          294-8
        }
}
The purpose of this study was to determine the effect of He-Ne laser irradiation (632.8 nm, 10 mW) on the induction of acrosome reaction and mortality in bull sperm cells in comparison with two important capacitation agents; calcium and heparin. Frozen-thawed bull sperm cells were washed in percoll gradient and suspended at a concentration of 1 x 10(6) ml-1 in sp-TALP medium, capacitated in the presence of 2 mM CaCl2, 10 micrograms ml-1 heparin, or irradiated at fluences from 2 to 16 J cm-2… CONTINUE READING

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