Biological consequences of DNA damage introduced in bacteriophage PM2 DNA by hydrogen peroxide-mediated free radical reactions.

@article{Gille1996BiologicalCO,
  title={Biological consequences of DNA damage introduced in bacteriophage PM2 DNA by hydrogen peroxide-mediated free radical reactions.},
  author={Johannes J. P. Gille and N M Wientjes and M. Vincent M. Lafleur and Hans Joenje and J. Ret{\`e}l},
  journal={Carcinogenesis},
  year={1996},
  volume={17 1},
  pages={5-11}
}
In order to study the biological consequences of DNA damage induced by H2O2-mediated free radical reactions, DNA from bacteriophage PM2 was exposed to H2O2, Fe(3+)-citrate and ascorbate either alone or in combination. Induction of DNA lesions was determined as well as the biological activity of the phage DNA. Exposure to H2O2 alone resulted in max. 0.2 single-strand breaks per molecule; in the presence of Fe(3+)-citrate, the yield was approximately 4-fold higher. Under both conditions no double… CONTINUE READING
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