Biological characterization of Fusobacterium necrophorum. Cell fractions in preparation for toxin and immunization studies.

@article{Garcia1975BiologicalCO,
  title={Biological characterization of Fusobacterium necrophorum. Cell fractions in preparation for toxin and immunization studies.},
  author={Manuel Moreno Garcia and David C. Alexander and K. A. MCKAY},
  journal={Infection and immunity},
  year={1975},
  volume={11 4},
  pages={609-16}
}
Fusobacterium necrophorum isolated from bovine liver abscesses was grown in bulk at 37 C for 24 h under a strict anaerobic atmosphere. Harvested washed cells were disrupted ultrasonically and fractionated by differential centrifugation into the intracellular (cytoplasm) and cell wall fractions. Both intact cells and cell fractions induced generalized cytopathic effect on primary pig kidney cultures and caused a variety of signs of illness and/or death of intraperitoneally injected mice. The… CONTINUE READING

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…